Anti-SSX2IP Rabbit Monoclonal Antibody

Anti-SSX2IP Rabbit Monoclonal Antibody

• Description: Boster Bio Anti-SSX2IP Rabbit Monoclonal Antibody catalog # M05918-1. Tested in WB, IHC applications. This antibody reacts with Human.
• Synonyms: Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein ;GM130 ;GM130 autoantigen ;Golgin-95 ;GOLGA2;
• CAS Number: 9007-83-4
• Gene Name: Golgin subfamily A member 2
• UniProt: Q9Y2D8
• Host: Rabbit
• Reactivity: Human
• Immunogen: A synthesized peptide derived from human SSX2IP
• Clonality: Monoclonal
• Clone: Clone: 30S65
• Applications: WB,IHC
• Purification: Affinity-chromatography
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Function: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin- alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401). .
• Storage Conditions: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 113086 MW
• Observed Molecular Weight: 74,76 kDa
• Fragment: IgG
• Applications Notes: WB 1:500-1:2000
  IHC 1:50-1:200