Anti-CD27 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-CD27 Rabbit Monoclonal Antibody catalog # M01148-3. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.

• Synonyms:

  NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA, MAD3, NFKBI

• Gene Name:

  NF-kappa-B inhibitor alpha

• UniProt:

  P26842

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  A synthesized peptide derived from human CD27

• Clonality:

  Monoclonal

• Clone:

  Clone: 20C54

• Tissue Specificity:

  Expressed in brain and skeletal muscle. .

• Applications:

  WB, ICC, IF, Flow Cytometry

• Field of Research:

  Chromatin Binding Proteins, Epigenetics and Nuclear Signaling

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG

• Applications Notes:

  WB 1:500-1:2000
  ICC/IF 1:50-1:200
  FC 1:50

• Other Gene Names:

  CD27

• Subcellular Location:

  Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. .

• Protein Name:

  Glycogen synthase kinase-3 beta (gsk3b)