Rat Endostatin PicoKine® Quick ELISA Kit

Rat Endostatin PicoKine® Quick ELISA Kit

• Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
• Description: Rat Endostatin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: Col18a1
• Gene ID: 85251
• UniProt: Q9WUW5
• Reactivity: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: H1591-K1774
• Applications: ELISA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <15 pg/ml
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	266	249	277	241	258	11.62	4.5%
  Sample 2	1674	1741	1852	1845	1778	99.57	5.6%
  Sample 3	4352	4333	4512	4026	4306	167.92	3.9%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant rat Col18a1