Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit

Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit

• Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
• Description: Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: Fcgr2b/Fcgr3
• Gene ID: 14130/14131
• UniProt: P08101/P08508
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: T40-R217
• Applications: ELISA
• Standard: 10 ng/tube
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Reproducibility

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	88	87	94	85	89	4.69	5.3%
  Sample 2	208	235	216	244	226	11.06	4.9%
  Sample 3	904	867	905	841	879	39.57	4.5%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant Mouse Fcgr2b/Fcgr3