Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit

Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit

• Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
• Description: Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Complement Factor H/CFH in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: Cfh
• Gene ID: 12628
• UniProt: P06909
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S875-V1252
• Applications: ELISA
• Standard: 100 ng/tube
• Detection Range: 0.78 ng/ml - 50 ng/ml
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <20 pg/ml
• Components: Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Reproducibility

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1344	1258	1323	1429	1339	84.33	6.3%
  Sample 2	6741	6541	5974	6641	6474	407.88	6.3%
  Sample 3	29641	26474	24165	26852	26783	1553.41	5.8%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant Mouse Cfh