Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit

Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit

• Background: KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
• Description: Mouse KIM1/TIM1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: hepatitis A virus cellular receptor 1
• Gene ID: 171283
• UniProt: Q5QNS5
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Y22-T212
• Applications: ELISA
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• : heparin or EDTA
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Sensitivity: <2 pg/ml
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	83	91	91	99	91	3.19	3.5%
  Sample 2	296	269	241	277	271	13.81	5.1%
  Sample 3	863	722	826	797	802	42.51	5.3%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant Mouse Havcr1