Luciferase firefly

CAT: 0749-PRP3004-01Size: 5 mgDry Ice: NoHazardous: No
CAT#:0749-PRP3004-01Size:5 mg
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Background
Firefly Luciferase catalyzes the reaction of luciferin with ATP and results in the production of yellow-green light. The enzyme has a molecular weight of 62 kDa and is expressed in peroxisomes. Luciferase is considered a model forstudying protein-anesthetic interactions. Firefly Luciferase is very useful in cell biology and molecular biology as a reporter of gene function and for the quantification of ATP
Description
Firefly Luciferase, Preparation method: E. coli
Tag
Firefly Luciferase
Type
Cytokines and proteins
Applications
His tag
Purity
> 98 % as determined by SDS-PAGE
Activity
>109 RLU/mg protein as determined in the presence of ATP and luciferin.
Molecular Weight
62 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Firefly Luciferase product can be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles
Protein Length
The recombinant Firefly Luciferase predicts a molecular mass of 62 kDa.
Formulation
Liquid in from sterile 20 mM Tris, 150 mM NaCl, 1mM DTT, pH 8.0
Other Product Names
Luciferase; Firefly Luciferase
Recommended Usage
Please ensure that the product is frozen before proceeding. Dissolve slowly at 4°C before use, and then proceed with other operations. If dilution is required, you can use a solution of 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8.0 to dilute to the specified concentration. For long-term storage, it is recommended to add 0.1% HSA or BSA or 5% trehalose
Preparation
E. coli
AA Sequence
Amino acid sequence of luciferase derived from Photinus pyralis (firefly) was expressed with 6xHis tag at the N-terminus.

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