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UNG Heat Labile

CAT: 0013-GTR19037407-01Size: 100 UDry Ice: NoHazardous: No
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CAT#:0013-GTR19037407-01Size:100 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Psychrophilic Marine Bacterium Uracil DNA Glycosylase, Heat Labile
Product Name Alternative
UDG, b2580, JW2564, EC 3.2.2.27, DGU, UNG15, HIGM5, Uracil-DNA Glycosylase 1, EC 3.2.2, HIGM4, UNG2.
Source
Escherichia Coli
Field of Research
Epigenetics & Chromatin
Purity
Greater than 97.0% as determined by SDS-PAGE.
Form
Sterile Filtered clear solution.
Storage Conditions
Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles
Notes
For research use only.
Applications Notes
Applications: Treatment of 0.1μg of uracil containing DNA with 1U UDG for 10 min. at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform. Unit Definition: 1 unit is defined as the amount of enzyme that releases 1 nmol of uracils from the DNA strand (containing dU) within 1 hour at 37°C in the reaction system containing 70mM TrisHCl, pH-7.5, 10mM NaCl, 1mM EDTA and 0.1mg/ml BSA reaction liquid
Preservative
UNG protein solution (1U/ul) 20mM Tris-HCl (25°C, pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP-40, 0.5% Tween-20 and 50% glycerol.

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