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Recombinant Hypoderma lineatum Collagenase

CAT: 0399-CSB-EP357515HRQ(M)-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP357515HRQ(M)-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
UniProt
P08897
Expression Region
31-260aa
Organism
Hypoderma lineatum (Early cattle grub) (Common cattle grub)
Target Sequence
IINGYEAYTGLFPYQAGLDITLQDQRRVWCGGSLIDNKWILTAAHCVHDAVSVVVYLGSAVQYEGEAVVNSERIISHSMFNPDTYLNDVALIKIPHVEYTDNIQPIRLPSGEELNNKFENIWATVSGWGQSNTDTVILQYTYNLVIDNDRCAQEYPPGIIVESTICGDTSDGKSPCFGDSGGPFVLSDKNLLIGVVSFVSGAGCESGKPVGFSRVTSYMDWIQQNTGIIF
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Source
E.coli
Field of Research
Others
Assay Type
In Stock Protein
Relevance
This enzyme is a serine protease capable of degrading the native triple helix of collagen. Also cleaves the B chain of insulin at the 15-Leu-|-Try-16 and 22-Arg-|-Gly-23 bonds. Hydrolyzes casein, but not Px-Pro-Leu-Gly-Pro-DArg, BzArgNHPh, AcTyrNHPh, 2-naphthyl phosphate, 2-naphthyl butyrate, 2-naphthyl caprylate, 2-naphthyl myristate, L-leucine 2-2-naphthylamide, L-valine 2-naphthylamide, L-cysteine 2-naphthylamide or L-glutarylphenylalanine 2-naphthylamide.2 Publications
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Length
Full Length of Mature Protein
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
32.7 kDa
References & Citations
"Sequential homology of the collagenase from eucaryote Hypoderma lineatum with the proteinases of the trypsin family." Lecroisey A., De Wolf A., Keil B. Biochem. Biophys. Res. Commun. 94:1261-1265 (1980)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

UniProtKB · P08897

Collagenase

COGS_HYPLI · Hypoderma lineatum

View on UniProt ↗
Primary accession
P08897
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Hypoderma lineatum (Early cattle grub)
Taxonomy ID
7389
Alternative names
—
EC number
3.4.21.49
Processing
Precursor
Secondary accessions
Q25083, Q9BPQ4
Protein keywords

Technical term

3D-structureDirect protein sequencing

Biological process

Collagen degradation

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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