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HOP Protein

CAT: 0013-GTR19030449-01Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19030449-01Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human Recombinant HOP Protein
Product Name Alternative
HOP, HSP co chaperone, STIP1, Stress-induced-phosphoprotein 1, Hsp70/Hsp90-organizing
UniProt
P31948
Reactivity
Human
Tag
His tag
Source
Recombinant
Origin
Human
Field of Research
Cancer Biology, Cell Biology
Concentration
Lot/batch specific. See included datasheet.
Purity
>90%
Molecular Weight
~63 kDa
Storage Conditions
-20°C
Notes
For research use only.
Applications Notes
This product has been certified >90% pure using SDS PAGE analysis. 4uM orb1714244, when added to 2uM (Aha1) -activated HSP90 (2uM; His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm) .
Tested Applications
FA, SDS-PAGE, WB
Preservative
25mM HEPES pH 7.2, 50mM NaCl, 1mM DTT, 10% glycerol
AA Sequence
MGHHHHHHMEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

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