Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)

Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)

• Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Abbreviation: Spike
• Synonyms: MERS-CoV S glycoprotein, MERS-CoV S protein, MERS-CoV S-trimer Protein, MERS-CoV Spike glycoprotein
• CAS Number: 9000-83-3
• Accession Number: AFS88936.1
• Host: HEK293 Cells
• Origin Species: MERS
• Tag: C-His
• Sequence: Tyr18-Lys1294(R751S)
• Applications: ELISA
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized MERS-CoV S-trimer Protein (R751S)-His(PKSV030287) at 5μg/ml (100 μl/well) can bind Human CD26-Fc(PKSH033696), The ED50 of Human CD26-Fc(PKSH033696) is 27.16 ng/ml.
• Reconstitution: Not Applicable
• Molecular Weight: 170-220 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
• Storage Conditions: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.