Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)

• Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
• Synonyms: E protein, Zika virus, strain Zika SPH2015
• CAS Number: 9000-83-3
• Accession Number: ALU33341.1
• Host: E. coli
• Origin Species: ZIKV
• Tag: N-His-MBP
• Sequence: Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592
• Purity: > 75 % as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.