Recombinant S. cerevisiae TIM16 Protein

• Background:

  Mitochondrial import inner membrane translocase subunit TIM16 (TIM16) is an ssential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM16 is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. TIM16 may act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.

• Abbreviation:

  TIM16

• Synonyms:

  Mitochondrial import inner membrane translocase subunit TIM16; PAM16; Presequence translocated-associated motor subunit PAM16; TIM16

• UniProt:

  P42949

• Accession Number:

  P42949

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Thr54-Ala119

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  7.9 kDa

• Observed Molecular Weight:

  11 kDa

• Species:

  S. cerevisiae