Recombinant Mouse G-CSF protein(N-His)

Recombinant Mouse G-CSF protein(N-His)

• Background: Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent.
• Abbreviation: G-CSF
• Synonyms: OPGL, CD254, ODF (Osteoclast Differentiation Factor), TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), sRNAK Ligand, soluble Receptor Activator of NF-kB Ligand
• CAS Number: 9000-83-3
• UniProt: P09920
• Accession Number: P09920
• Host: E. coli
• Origin Species: Mouse
• Tag: N-His
• Sequence: Val 31-Ala 208
• Applications: Cell culture
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant mouse G-CSF is > 2 x 10⁷ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 17-25 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.