Recombinant Mouse FGCP/GCPII/mGCP/NAALADase I/PSMA (N-6His)

• Background:

  Glutamate carboxypeptidase 2, also known as FOLH1, PSMA, belongs to the M28B subfamily and the peptidase M28 family. It is highly expressed in prostate epithelium and can be detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level) . PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease. It has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity and has a preference for tri-alpha-glutamate peptides. PSMA involves in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, PSMA cleaves Gly-Pro-AMC. PSMA is stable at pH greater than 6.5.

• Abbreviation:

  FGCP, GCPII, mGCP, PSMA

• Synonyms:

  Cell growth-inhibiting gene 27 protein; FGCP; Folate hydrolase 1; GCPII; Glutamate carboxypeptidase 2; NAALADase I; PSMA; mGCP

• UniProt:

  Q0VDM5

• Accession Number:

  Q0VDM5

• Expression System:

  HEK293 Cells

• Tag:

  N-His

• Sequence:

  Ile44-Ala752

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  80.7 kDa

• Observed Molecular Weight:

  100 - 120 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 8% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH7.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse