Recombinant Mouse IGF1(Long-R3-IGF1) Protein (His Tag)

Recombinant Mouse IGF1(Long-R3-IGF1) Protein (His Tag)

• Background: Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides. These isoforms are differentially expressed by various tissues. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively, and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II. IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. It plays an important role in muscle regeneration and tumor progression. IGF-I binds IGF-I R, IGF-II R, and the insulin receptor. IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors.
• Abbreviation: IGF1
• Synonyms: IGF-1, IGF1, Insulin-like growth factor I, Somatomedin C, insulin-like growth factor 1, somatomedin-C
• UniProt: P05017
• Accession Number: P05017
• Host: E. coli
• Origin Species: Mouse
• Tag: C-His
• Sequence: Gly49-Ala118
• Applications: Cell culture
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <2 ng/mL. The specific activity of recombinant mouse IGF-I is > 5 x 10⁵ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 11-17 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.