Recombinant Mouse MMP12/MMP-12/HME Protein (His Tag)

• Background:

  Matrix metalloproteinase-12 (MMP12) is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family. MMP12 may be involved in tissue injury and remodeling and have significant elastolytic activity. It can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

• Abbreviation:

  MMP12, MMP-12, HME

• Synonyms:

  MME; MMP-12; Macrophage metalloelastase; Matrix metalloproteinase-12; Mme; Mmel; Mmp12

• UniProt:

  P34960

• Accession Number:

  P34960

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Ala29-Cys473

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  53.0 kDa

• Observed Molecular Weight:

  64 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 20mM PB, 500mM NaCl, 0.01%Tween 80,1mM EDTA, 50% Glycerol, pH6.0.

• Species:

  Mouse