Recombinant Mouse Interleukin-33/IL-33 Protein

Recombinant Mouse Interleukin-33/IL-33 Protein

• Background: Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.
• Abbreviation: Interleukin-33;IL-33
• Synonyms: DVS, NF-HEV and IL- 1F, Interleukin-1 family member, C9orf, C9orf26, DVS27, Interleukin-1 family member 11, NF-HEV and IL- 1F11, NKHEV, IL33, Interleukin-33, IL-33, Interleukin 33, IL1F11, NFEHEV, NF-HEV
• CAS Number: 9000-83-3
• UniProt: Q8BVZ5
• Accession Number: Q8BVZ5
• Host: E. coli
• Origin Species: Mouse
• Tag: C-His
• Sequence: Ser109-Ile266
• Applications: Cell culture
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce proliferation in D10.G4.1 cells. The ED₅₀ for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10⁷ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 17-25 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.