Recombinant Mouse Interleukin-22/IL-22 Protein (mFc Tag)

• Background:

  Interleukin-22 (IL-22) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acid signal peptide that is cleaved to generate a 147 amino acid mature protein that shares approximately 79% and 22% sequence identity with human IL22 and IL10, respectively. IL22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and up-regulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.

• Abbreviation:

  Interleukin-22, IL-22

• Synonyms:

  Interleukin; Cytokine Zcyto; IL-D; TIFIL; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-22a; IL-D110; IL-TIF; Interleukin-22a; TIFa; TIFIL-23; UNQ3099/PRO10096; zcyto18; Interleukin-22; T-cell-derived-inducible; factor (IL-TIF) ; Interleukin-22a (IL-22a) ; IL-TIF alpha; IL-10-related; IL-22; Il22a; Iltif; Iltifa; Il22; TIFa

• UniProt:

  Q9JJY9

• Accession Number:

  Q9JJY9

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc

• Sequence:

  Leu34-Val179

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  43.8 kDa

• Observed Molecular Weight:

  50 - 65 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse