Recombinant Mouse IL-1RA/IL1RN Protein

• Background:

  Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1 beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouse Il1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26% and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.

• Abbreviation:

  IL-1RA; IL1RN

• Synonyms:

  IL1F; MVCD; IL-1ra; F630041P17Rik; DIRA; ICIL-1RA; IL1 inhibitor; IL1F3; IL1RA; IL-1ra3; IL-1RN; Interleukin-1 Receptor Antagonist Protein; IRAP; MVCD4; Il1rn; F630041P17Rik

• UniProt:

  P25085

• Accession Number:

  P25085

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Arg27-Gln178

• Applications:

  Cell culture

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.3 kDa

• Observed Molecular Weight:

  17 kDa

• Species:

  Mouse