Recombinant Mouse Nogo Receptor/NgR Protein (His Tag)

Recombinant Mouse Nogo Receptor/NgR Protein (His Tag)

• Background: Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.
• Synonyms: NOGOR, NgR, NgR1, Rtn4r
• CAS Number: 9000-83-3
• UniProt: Q99PI8
• Accession Number: NP_075358.1
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His
• Sequence: Met 1-Ser 447
• Applications: ELISA
• Purity: > 97 % as determined by reducing SDS-PAGE.
• Bioactivity: 1. Immobilized recombinant Mouse RTN4R at 2 μg/ml (100 μl/well) can bind biotinylated human RTN4 (GST Tag) with a linear range of 0.04-0.625 μg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 65 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.