Recombinant Mouse REG4/RELP Protein (His Tag)

Recombinant Mouse REG4/RELP Protein (His Tag)

• Background: Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.
• Synonyms: 2010002L15Rik, GISP, RELP
• CAS Number: 9000-83-3
• UniProt: Q9D8G5
• Accession Number: NP_080604.2
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His
• Sequence: Met 1-Thr 157
• Applications: Cell culture
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 10⁴ cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), > 70% cell will adhere after 60 minutes at 37°C.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 23-30 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.