Recombinant Mouse LRPAP1/A2MRAP Protein (His Tag)

Recombinant Mouse LRPAP1/A2MRAP Protein (His Tag)

• Background: Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2, and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin.
• Synonyms: AA617339, AI790446, AU042172, C77774, HBP44, RAP
• CAS Number: 9000-83-3
• UniProt: P55302
• Accession Number: NP_038615.2
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: C-His
• Sequence: Gln 29-Leu 360
• Applications: ELISA
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Immobilized mouse LRPAP1 at 0.5 μg/ml can bind human VLDLR with a linear range of 1. 28-32 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 46 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.