Recombinant Human Transforming Growth Factor Beta-1/TGFB1 Biotinylated

• Background:

  Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA) . TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.

• Abbreviation:

  TGFB1

• Synonyms:

  LAP; Latency-Associated Peptide; TGF-Beta-1; TGFB; TGFB1; Transforming Growth Factor Beta-1

• UniProt:

  P01137

• Accession Number:

  P01137

• Expression System:

  HEK293 Cells

• Conjugation:

  Biotinylated

• Tag:

  N-Avi

• Sequence:

  Ala279-Ser390

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cell biology; Cardiovascular; Cancer; metabolism; Stem cells

• Endotoxin:

  < 0.01 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED₅₀ for this effect is 137.37pg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  14.6 kDa

• Observed Molecular Weight:

  12 - 18 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH2.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human