Recombinant Human SLAM Family Member 7/SLAMF7/CD319/CRACC (C-mFc)

• Background:

  SLAMF7 is a single-pass type I membrane protein and contains 1 Ig-like C2-type (immunoglobulin-like) domain. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM) -associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, SLAMF7 can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.

• Abbreviation:

  SLAMF7, CD319, CRACC

• Synonyms:

  UNQ; PRO; SLAM; SLAMF7;19A; CD319; CRACC; CS1;19A24 protein; CD 319; CD2 like receptor activating cytotoxic cells; CD2 subset 1; CD2-Like Receptor-Activating Cytotoxic Cells; CD319 antigen; Membrane protein FOAP 12; Membrane Protein FOAP-12; Novel Ly9; Novel LY9 (lymphocyte antigen 9) like protein; Protein 19A; SLAM family member 7; SLAM7; SLAMF 7; UNQ576; PRO1138; CS1; CD319; CRACC; CS1; Novel Ly9

• UniProt:

  Q9NQ25

• Accession Number:

  Q9NQ25

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc

• Sequence:

  Ser23-Met226

• Field of Research:

  Immunology

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  49.0 kDa

• Observed Molecular Weight:

  60 - 90 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human