Recombinant Human BCL2L1/Bcl-XL Protein (His Tag)

• Background:

  Bcl-2-Like Protein 1 (BCL2L1) is a member of the Bcl-2 family. BCL2L1 is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. BCL2L1 is a mitochondrial membrane protein. BCL2L1 contains four motifs, BH1, BH2 and BH4. The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. BCL2L1 regulates cell death by blocking the voltage-dependent anion channnel (VDAC) and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. In addition, BCL2L1 promotes apoptosis.

• Abbreviation:

  BCL2L1; Bcl-XL

• Synonyms:

  Apoptosis Regulator Bcl-X; BCL-XL/S; BCL2L; BCLX; BCLXL; BCLXS; Bcl-2-Like Protein 1; Bcl-X; Bcl2-L-1; PPP1R52; bcl-xL; bcl-xS

• UniProt:

  Q07817

• Accession Number:

  Q07817

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Met1-Arg212

• Field of Research:

  Signal Transduction; Cell biology; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  24.9 kDa

• Observed Molecular Weight:

  29 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM HEPES, 50mM KCl, 20% Glycerol, pH 7.5.

• Species:

  Human