Recombinant Human Interleukin-7/IL-7 Protein

• Background:

  Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.

• Abbreviation:

  Interleukin-7; IL-7

• Synonyms:

  Lymphopoietin; IL7; IL-7; IL 7; Interleukin 7; Interleukin-7; LP-1; Lymphopoietin 1

• UniProt:

  P13232

• Accession Number:

  P13232

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Asp26-His177

• Applications:

  Cell culture

• Field of Research:

  Immunology

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50 1 x 108 IU/mg.8

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  18.3 kDa

• Observed Molecular Weight:

  19 kDa

• Species:

  Human