Recombinant Human Interleukin-7/IL-7 Protein

Recombinant Human Interleukin-7/IL-7 Protein

• Background: Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
• Abbreviation: Interleukin-7;IL-7
• Synonyms: Lymphopoietin, IL7, IL-7, IL 7, Interleukin 7, Interleukin-7, LP-1, Lymphopoietin 1
• CAS Number: 9000-83-3
• UniProt: P13232
• Accession Number: P13232
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Asp26-His177
• Applications: Cell culture
• Field of Research: immunology
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED₅₀ for this effect is <0.8 ng/mL. The specific activity of recombinant human IL-7 is > 1 x 10⁸ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 19 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.