Recombinant Human BDNF Protein (with pro peptide)

• Background:

  The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

• Abbreviation:

  BDNF

• Synonyms:

  Abrineurin; BDNF; Brain-Derived Neurotrophic Factor

• UniProt:

  P23560

• Accession Number:

  P23560

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Ala19-Arg247 (R125A, R127A, R128A)

• Field of Research:

  Cardiovascular; Neuroscience; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  25.6 kDa

• Observed Molecular Weight:

  28 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0.

• Species:

  Human