Recombinant Human Interleukin-20/IL-20 Protein

Recombinant Human Interleukin-20/IL-20 Protein

• Background: Interleukin-20 (IL-20) is a member of the IL-10 family of regulatory cytokines that includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences but they are dissimilar in their biological functions. IL-20 exhibits approximately 28% amino acid identity with IL-10 and 76% amino acid identity with mouse IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 Rα and IL-20 Rβ. The second is composed of IL-22 R and IL-20 Rβ. Whereas the IL-22 R/IL-20 Rβ complex is shared with IL-24, the IL-20 Rα/IL-20 Rβ complex is shared with both IL-19 and IL-24. IL-20 has been shown to initiate transduction cascades involving STAT3 and stimulates the induction of pro-inflammatory genes including TNF-α and MCP-1. Initial functional studies using transgenic mice suggest that IL-20 has the ability to regulate skin development. The over-expression of both human and mouse forms of IL-20 results in keratinocyte hyper-proliferation, abnormal epidermal differentiation, and neonatal lethality. In humans, IL-20 and its receptors are up-regulated in psoriatic skin, and polymorphisms in the IL-20 gene have been associated with plaque-type psoriasis. IL-20 may also have a role in hematopoiesis. It enhances the proliferation of multi-potential progenitors in vitro and increases their numbers and cell cycling status in IL-20 transgenic mice. IL-20 is also shown to suppress COX-2 and PGE2 and acts as an inhibitor of angiogenesis in model systems.
• Abbreviation: Interleukin-20;IL-20
• Synonyms: UNQ, PRO, Interleukin, Cytokine Zcyto, ZCYTO, IL20, IL-20, IL10D, ZCYTO10, Cytokine Zcyto10, Interleukin-20, UNQ852, PRO1801
• CAS Number: 9000-83-3
• UniProt: Q9NYY1
• Accession Number: Q9NYY1
• Host: E. coli
• Origin Species: Human
• Tag: C-His
• Sequence: Leu25-Glu176
• Applications: Cell culture
• Field of Research: immunology
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to chemoattract BaF3 cells transfected with human IL-20R alpha and IL-20R beta. The ED₅₀ for this effect is <0.2 ng/mL.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 16 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.