Recombinant Human Apolipoprotein A-I/ApoAI Protein

• Background:

  Apolipoprotein A1 (APOA1) is a secreted protein which belongs to the Apolipoprotein A1/A4/E family. APOA1 is the major protein component of high density lipoprotein (HDL) in plasma. APOA1 plays a critical role in various biological processes; such as Cholesterol metabolism; Lipid metabolism and transport; Steroid metabolism. APOA1 promotes cholesterol efflux from tissues to the liver and thus helps to clear cholesterol from arteries. Defects in this gene resulted in HDL deficiencies; including Tangier disease (TGD) ; systemic non-neuropathic amyloidosis; premature coronary artery disease; hepatosplenomegaly and progressive muscle wasting and weakness. In addition; ApoA-I is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

• Abbreviation:

  Apolipoprotein A-I; ApoAI

• Synonyms:

  APOA1; Apo-AI; ApoA-I; Apolipoprotein A-I; Apolipoprotein A1

• UniProt:

  P02647

• Accession Number:

  P02647

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Arg19-Gln267

• Field of Research:

  Signal Transduction; Cardiovascular; Neuroscience; Cancer; metabolism

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  29.0 kDa

• Observed Molecular Weight:

  25-31 kDa

• Species:

  Human