Recombinant Human G-CSF/CSF3 Protein (Fc Tag)

Recombinant Human G-CSF/CSF3 Protein (Fc Tag)

• Background: Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.
• Synonyms: C17orf33, CSF3, Filgrastim, G-CSF, GCSF, Granulocyte Colony-Stimulating Factor, Lenograstim, Pluripoietin
• CAS Number: 9000-83-3
• UniProt: P09919
• Accession Number: NP_757373.1
• Host: HEK293 Cells
• Origin Species: Human
• Tag: N-hFc
• Sequence: Ala 30-Pro 204
• Applications: Cell culture
• Field of Research: Cancer;immunology
• Purity: > 97 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.The ED50 for this effect is typically 0.2-0.8 ng/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 48 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.