Recombinant Human IL1R1/CD121a Protein

• Background:

  Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA) . IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

• Abbreviation:

  IL1R1/CD121a

• Synonyms:

  D2S; Interleukin-1 receptor type; IL1RT; IL1-R; IL1R1; CD121A; D2S1473; IL-1R-alpha; IL1R; IL1RA; P80; CD121 antigen-like family member A; IL-1 RI; IL1-R1; IL-1R1; IL-1R-1; IL-1RT1; IL-1RT-1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; IL1RT1; IL-1R type I; IL-1RI

• UniProt:

  P14778

• Accession Number:

  NP_000868.1

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Met 1-Thr332

• Field of Research:

  Cardiovascular; immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  36.8 kDa

• Observed Molecular Weight:

  56 kDa

• Species:

  Human