Recombinant Human Cystatin 7/CST7 Protein (Fc Tag)

• Background:

  The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

• Synonyms:

  CMAP; CST7; Cystatin-7; Cystatin-F; Cystatin-Like Metastasis-Associated Protein; Leukocystatin

• UniProt:

  O76096

• Accession Number:

  NP_003641.3

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Sequence:

  Met 1-His 145

• Applications:

  Enzyme

• Field of Research:

  Cell biology; Cardiovascular

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog#ES008) . The IC50 value is < 5 nM.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  41.3 kDa

• Observed Molecular Weight:

  50 kDa

• Species:

  Human