Recombinant Human STAT6 Protein (Baculovirus, His Tag)

• Background:

  Signal transducer and activator of transcription 6 (STAT6) is a transcription factor that is activated by interleukin-4 (IL-4) -induced tyrosine phosphorylation and mediates most of the IL-4-induced gene expression. STAT6 plays a central role in exerting interleukin-4 (IL-4) mediated biological responses and is found to induce the expression of BCL2L1/BCL-XL, which is responsible for the anti-apoptotic activity of IL4. Transcriptional activation by STAT6 requires the interaction with coactivators like p300 and the CREB-binding protein (CBP) . NF-κB and tyrosine-phosphorylated Stat6 can directly bind each other in vitro and in vivo, which suggests that the direct interaction between Stat6 and NF-κB may provide a basis for synergistic activation of transcription by IL-4 and activators of NF-κB.

• Synonyms:

  IL-4 Stat; IL-4-STAT; STAT6; STAT6B; STAT6C; Signal Transducer and Activator of Transcription 6

• UniProt:

  P42226

• Accession Number:

  P42226-1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  C-His

• Sequence:

  Met 1-Trp 847

• Field of Research:

  Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Kits, Lysates, Other

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  95.5 kDa

• Observed Molecular Weight:

  100 kDa

• Species:

  Human