Recombinant Human Thioredoxin/TXN Protein

• Background:

  Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to thethioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO) ; and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

• Synonyms:

  ADF; ATL-Derived Factor; SASP; Surface-Associated Sulphydryl Protein; TRDX; TRX; TRX1; TXN; Thioredoxin; Trx

• UniProt:

  P10599

• Accession Number:

  P10599

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Met 1-Val 105

• Applications:

  Enzyme

• Field of Research:

  Signal Transduction; metabolism;

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 97 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  11.7 kDa

• Observed Molecular Weight:

  14 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.5
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human