Recombinant Human WISP1 protein (His tag)

Recombinant Human WISP1 protein (His tag)

• Background: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.
• Abbreviation: WISP1
• Synonyms: CCN family member 4, CCN4, WISP-1, WISP1, WNT1-inducible-signaling pathway protein 1, Wnt-1-induced secreted protein
• CAS Number: 9000-83-3
• UniProt: O95388
• Accession Number: O95388
• Host: HEK293 Cells
• Origin Species: Human
• Tag: C-His
• Sequence: Met1-Asn367
• Field of Research: Epigenetics and Nuclear Signaling
• Purity: > 95% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
• Molecular Weight: 60 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.