Luciferase from Photinus pyralis (firefly)

• Description:

  Luciferase from Photinus pyralis (firefly)_x000D_ Catalog number: B2016650_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 500 ug_x000D_ Molecular Weight or Concentration: 62 kDa_x000D_ Supplied as: Solution_x000D_ Applications: a molecular tool for various biochemical applications_x000D_ Storage: ˆ’20°C_x000D_ Keywords: Luciferase firefly_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References: _x000D_ 1: Smale ST. Luciferase assay Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot5421. _x000D_ 2: de Wet JR, Wood KV, DeLuca M, Helinski DR, Subramani S. Firefly luciferase gene: structure and expression in mammalian cells Mol Cell Biol. 1987 Feb;7(2):725-37. _x000D_ 3: Branchini BR, Southworth TL, Fontaine DM, Davis AL, Behney CE, Murtiashaw MH. A Photinus pyralis and Luciola italica chimeric firefly luciferase produces enhanced bioluminescence Biochemistry. 2014 Oct 14;53(40):6287-9. _x000D_ 4: Hall MP, Unch J, Binkowski BF, Valley MP, Butler BL, Wood MG, Otto P, Zimmerman K, Vidugiris G, Machleidt T, Robers MB, Benink HA, Eggers CT, Slater MR, Meisenheimer PL, Klaubert DH, Fan F, Encell LP, Wood KV. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate ACS Chem Biol. 2012 Nov 16;7(11):1848-57. _x000D_ 5: Zhang H. HSV1-TK/GFP/Fluc 2008 Oct 21 [updated 2008 Dec 1]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004€“2013._x000D_ 6: Smith AD, Trempe JP. Luminometric quantitation of photinus pyralis firefly luciferase and Escherichia coli beta-galactosidase in blood-contaminated organ lysates Anal Biochem. 2000 Nov 1;286(1):164-72. _x000D_ 7: Herbst R, Schäfer U, Seckler R. Equilibrium intermediates in the reversible unfolding of firefly (Photinus_pyralis) luciferase J Biol Chem. 1997 Mar 14;272(11):7099-105. _x000D_ 8: Herbst R, Gast K, Seckler R. Folding of firefly (Photinus_pyralis) luciferase: aggregation and reactivation of unfolding intermediates Biochemistry. 1998 May 5;37(18):6586-97. _x000D_ 9: Conti E, Lloyd LF, Akins J, Franks NP, Brick P. Crystallization and preliminary diffraction studies of firefly luciferase from Photinus_pyralis Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):876-8. _x000D_ 10: Muthukumaran T, KrishnaMurthy NV, Sivaprasad N, Sudhaharan T. Isolation and characterization of luciferase from Indian firefly, Luciola praeusta Luminescence. 2014 Feb;29(1):20-8. _x000D_
  Products Related to Luciferase from Photinus_pyralis (firefly) can be found at Enzymes

• Short Description:

  Catalog Number: B2016650 (500 ug)_x000D_ Luciferase from Photinus pyralis (firefly) is a high quality enzyme found in fireflies, specifically in the species Photinus pyralis. It is responsible for the bioluminescence, or light production, that fireflies are known for. Luciferase catalyzes the reaction that produces light by oxidizing a substrate called luciferin in the presence of oxygen, ATP, and magnesium ions. This reaction results in the emission of light. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height :

  0.5