DNMT3B Polyclonal Antibody

• Background:

  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

• Abbreviation:

  DNMT3B

• UniProt:

  Q9UBC3

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  A synthetic peptide of human DNMT3B

• Target:

  DNMT3B; ICF; ICF1; M.HsaIIIB

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IF

• Field of Research:

  Cancer; Epigenetics and Nuclear Signaling

• Purification:

  Affinity purification

• Concentration:

  1mg/mL

• Dilution:

  WB 1:200-1:1000; IF 1:50-1:200

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  77-95kDa

• Observed Molecular Weight:

  110kDa

• Isotype:

  IgG