Beta actin Polyclonal Antibody

• Background:

  Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

• Abbreviation:

  ACTBB

• UniProt:

  Q7ZVF9

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant Zebrafish Actin, cytoplasmic 2 protein

• Target:

  ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Isotype; Tags and Cell markers; Signal Transduction

• Purification:

  Antigen Affinity Purification

• Concentration:

  0.85mg/mL

• Dilution:

  WB 1:3000-1:30000; IHC 1:100-1:300

• Buffer:

  PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  41 kDa

• Observed Molecular Weight:

  41 kDa

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S2162253120301724

• Citation 02:

  https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00114d

• Citation 03:

  https://www.mdpi.com/2073-4409/10/10/2647

• Citation 04:

  https://www.mdpi.com/2072-6694/13/9/2066

• Citation 05:

  https://www.mdpi.com/2073-4409/10/5/1013

• Citation 06:

  https://www.mdpi.com/1905846

• Citation 07:

  https://www.mdpi.com/1905046

• Citation 08:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7293

• Citation 09:

  https://www.sciencedirect.com/science/article/pii/S1567576922007597

• Citation 10:

  https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-1132-3

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S0944711321003883

• Citation 12:

  https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgac065/6726478

• Isotype:

  IgG