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Beta actin Polyclonal Antibody

CAT: 0763-E-AB-40338-01Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0763-E-AB-40338-01Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Abbreviation
ACTBB
UniProt
Q7ZVF9
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant Zebrafish Actin, cytoplasmic 2 protein
Target
ACTB; Actin beta; Actx; Actin cytoplasmic 1; b actin; A26C1A
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Isotype; Tags and Cell markers; Signal Transduction
Purification
Antigen Affinity Purification
Concentration
0.85mg/mL
Dilution
WB 1:3000-1:30000; IHC 1:100-1:300
Buffer
PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
41 kDa
Observed Molecular Weight
41 kDa
Citation 01
https://www.sciencedirect.com/science/article/pii/S2162253120301724
Citation 02
https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00114d
Citation 03
https://www.mdpi.com/2073-4409/10/10/2647
Citation 04
https://www.mdpi.com/2072-6694/13/9/2066
Citation 05
https://www.mdpi.com/2073-4409/10/5/1013
Citation 06
https://www.mdpi.com/1905846
Citation 07
https://www.mdpi.com/1905046
Citation 08
https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7293
Citation 09
https://www.sciencedirect.com/science/article/pii/S1567576922007597
Citation 10
https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-1132-3
Citation 11
https://www.sciencedirect.com/science/article/pii/S0944711321003883
Citation 12
https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgac065/6726478
Isotype
IgG

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