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GAPDH Polyclonal Antibody

CAT: 0763-E-AB-40337-01Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0763-E-AB-40337-01Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Abbreviation
GAPDH
UniProt
Q5XJ10
Host
Rabbit
Reactivity
Human; Mouse; Rat
Immunogen
Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
Target
Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Field of Research
Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism
Purification
Antigen Affinity Purification
Concentration
1 mg/mL
Dilution
WB 1:3000-1:6000; IHC 1:300-1:500
Buffer
PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
35 kDa
Observed Molecular Weight
35 kDa
Citation 01
https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btm2.10275
Citation 02
https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3919938
Citation 03
https://pubmed.ncbi.nlm.nih.gov/35860242/
Citation 04
https://www.sciencedirect.com/science/article/pii/S0753332222006783
Citation 05
https://www.mdpi.com/1579044
Citation 06
https://www.sciencedirect.com/science/article/pii/S0223523422003117
Citation 07
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019468/
Citation 08
https://www.sciencedirect.com/science/article/pii/S1567576922004763
Citation 09
https://www.sciencedirect.com/science/article/pii/S1567576923000449
Citation 10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053591/
Citation 11
https://iovs.arvojournals.org/article.aspx?articleid=2783840
Citation 12
https://www.sciencedirect.com/science/article/pii/S1567576921000928
Isotype
IgG

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