GAPDH Polyclonal Antibody

• Background:

  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

• Abbreviation:

  GAPDH

• UniProt:

  Q5XJ10

• Host:

  Rabbit

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

• Target:

  Aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism

• Purification:

  Antigen Affinity Purification

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:3000-1:6000; IHC 1:300-1:500

• Buffer:

  PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Observed Molecular Weight:

  35 kDa

• Citation 01:

  https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btm2.10275

• Citation 02:

  https://papers.ssrn.com/sol3/papers.cfm?abstract_id=3919938

• Citation 03:

  https://pubmed.ncbi.nlm.nih.gov/35860242/

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0753332222006783

• Citation 05:

  https://www.mdpi.com/1579044

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0223523422003117

• Citation 07:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019468/

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/S1567576922004763

• Citation 09:

  https://www.sciencedirect.com/science/article/pii/S1567576923000449

• Citation 10:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053591/

• Citation 11:

  https://iovs.arvojournals.org/article.aspx?articleid=2783840

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/S1567576921000928

• Isotype:

  IgG