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Flag-Tag Polyclonal Antibody

CAT: 0763-E-AB-20045-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0763-E-AB-20045-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
Abbreviation
Flag-Tag
Host
Rabbit
Immunogen
Synthetic Peptide
Target
DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB
Field of Research
Tags and Cell Markers
Purification
Affinity purification
Concentration
1 mg/mL
Dilution
WB 1:5000-20000
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0896841122001834
Isotype
IgG

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