HLA-DRB1 Polyclonal Antibody

• Background:

  HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

• Abbreviation:

  HLA-DRB1

• UniProt:

  Q5Y7A7

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Fusion protein of human HLA-DRB1

• Target:

  MHC Class II HLA-DR Beta 1 Chain; Human Leucocyte Antigen DRB1; MHC Class II Antigen DRB1*10; MHC Class II Antigen DRB1*11; MHC Class II Antigen DRB1*12; MHC Class II Antigen DRB1*13; MHC Class II Antigen DRB1*14; MHC Class II Antigen DRB1*15; MHC Class II Antigen DRB1*16; MHC Class II Antigen DRB1*1; MHC Class II Antigen DRB1*3; MHC Class II Antigen DRB1*4; MHC Class II Antigen DRB1*7; MHC Class II Antigen DRB1*8; MHC Class II Antigen DRB1*9; Lymphocyte Antigen DRB1c

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Immunology

• Purification:

  Antigen affinity purification

• Concentration:

  1.32 mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:50-1:300

• Buffer:

  PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  30 kDa

• Observed Molecular Weight:

  Refer to figures

• Isotype:

  IgG