HLA-B Polyclonal Antibody

• Background:

  HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

• Abbreviation:

  HLA-B

• UniProt:

  P01889

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide of human HLA-B

• Target:

  1B07; AS; B-4901; B-7 alpha chain; HC class I antigen SHCHA; HLA class I histocompatibility antigen; HLA class I histocompatibility antigen; B alpha chain; HLA-B; HLA-B histocompatibility type; HLAB; leukocyte antigen class I-B; major histocompatibility complex; class I; B; MHC class 1 antigen; MHC class I antigen B*7; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC Class I HLA heavy chain; MHC class I molecule; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; SPDA1

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC

• Field of Research:

  Immunology; Microbiology

• Purification:

  Antigen affinity purification

• Concentration:

  0.9 mg/mL

• Dilution:

  WB 1:500-1:2000; IHC 1:30-1:150

• Buffer:

  PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  40 kDa

• Observed Molecular Weight:

  Refer to figures

• Isotype:

  IgG