Glutamine (Gln) Colorimetric Assay Kit

• Target:

  Gln

• Type:

  Colorimetric method

• Field of Research:

  Amino Acids and Proteins; cuproptosis

• Assay Principle:

  Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.

• Assay Performance Time:

  70 min

• Sample Type:

  Serum (plasma) ; animal; plant tissue

• Detection Range:

  0.036–2.0 mmol/L

• Sensitivity:

  0.036 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4.7%

• Intra CV:

  3.2%

• Detection Instrument:

  Microplate reader