FoxO1A (Acetyl Lys245) rabbit pAb

FoxO1A (Acetyl Lys245) rabbit pAb

• Description: Disease: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM: 268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13) (q35;q14) with PAX3; translocation t (1;13) (p36;q14) with PAX7. The resulting protein is a transcriptional activator. function: Transcription factor. PTM: Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Contains 1 fork-head DNA-binding domain. subcellular location: Shuttles between cytoplasm and nucleus. subunit: Interacts with LRPPRC. tissue specificity: Ubiquitous.
• Synonyms: Forkhead box protein O1 (Forkhead box protein O1A;Forkhead in rhabdomyosarcoma)
• Gene ID: 2308
• UniProt: Q12778
• Cellular Locus: Cytoplasm. Nucleus. Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from human FoxO1A (Acetyl Lys245)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1000-2000 ELISA 1:5000-20000
• Molecular Weight: 72kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.