Succinate Dehydrogenase (SDH) Activity Assay Kit

• Target:

  SDH

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function; Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP) . Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.

• Assay Performance Time:

  10 min

• Sample Type:

  Animal tissue; cell

• Detection Range:

  0.83-65.42 U/L

• Sensitivity:

  0.83 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4%

• Intra CV:

  2%

• Detection Instrument:

  Microplate reader