Pyruvate Kinase (PK) Activity Assay Kit

• Target:

  PK

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.

• Assay Performance Time:

  10 min

• Sample Type:

  Serum; plasma; tissue; cell

• Detection Range:

  0.08–6.79 U/L

• Sensitivity:

  0.08 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  7%

• Intra CV:

  5%

• Detection Instrument:

  Microplate reader