Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)

• Target:

  BUN

• Type:

  Colorimetric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:

• Assay Performance Time:

  60 min

• Sample Type:

  Serum; plasma; saliva; urine

• Detection Range:

  0.12-15 mmol/L

• Sensitivity:

  0.12 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S1742706121007108

• Citation 02:

  https://www.hindawi.com/journals/omcl/2022/1763922/

• Citation 03:

  https://www.mdpi.com/2124124

• Citation 04:

  https://www.mdpi.com/article/10.3390/ijms232315400

• Citation 05:

  https://europepmc.org/article/med/35392552?javascript_support=no

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0753332220308775

• Citation 07:

  https://www.tandfonline.com/doi/abs/10.1080/09603123.2022.2105824

• Citation 08:

  https://www.frontiersin.org/articles/10.3389/fphys.2023.1083725/full?utm_source=dlvr.it&utm_medium=twitter

• Citation 09:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525417/

• Citation 10:

  https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgab078/6299670

• Citation 11:

  https://www.mdpi.com/1433254

• Inter CV:

  9.9%

• Intra CV:

  4.9%

• Detection Instrument:

  Spectrophotometer