Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method)

• Target:

  T-AOC

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress; Plant Physiology Series

• Assay Principle:

  The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

• Assay Performance Time:

  20 min

• Sample Type:

  Serum; plasma; urine; saliva; tissue; cells

• Detection Range:

  0.05-1.00 mmol/L

• Sensitivity:

  0.05 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.mdpi.com/1332228

• Citation 02:

  https://pubmed.ncbi.nlm.nih.gov/34776947/

• Citation 03:

  https://www.mdpi.com/article/10.3390/foods12040767

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/S0753332220308775

• Citation 05:

  https://www.nature.com/articles/s41598-021-91256-6

• Citation 06:

  https://www.dovepress.com/the-preventive-and-therapeutic-effects-of-ajwa-date-fruit-extract-agai-peer-reviewed-fulltext-article-DDDT

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/S037887412100893X

• Citation 08:

  https://www.tandfonline.com/doi/abs/10.1080/09205063.2022.2099668

• Citation 09:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.12010

• Citation 10:

  https://link.springer.com/article/10.1007/s10904-023-02532-z

• Citation 11:

  https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221

• Citation 12:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894603/

• Inter CV:

  5%

• Intra CV:

  4.1%

• Detection Instrument:

  Microplate reader