Adenosine Deaminase (ADA) Activity Assay Kit

• Target:

  ADA

• Type:

  Colorimetric method

• Field of Research:

  Liver and Renal Function

• Assay Principle:

  Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.

• Assay Performance Time:

  40 min

• Sample Type:

  Serum; plasma; animal tissue

• Detection Range:

  0.03-99 U/L

• Sensitivity:

  0.03 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm01836e

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S2405844021000256

• Inter CV:

  6%

• Intra CV:

  3%

• Detection Instrument:

  Microplate reader